Restrain proliferation of cervical cancer ( 12, 13). MiRNAs that were significantly altered and have the potential to miRNAs have been recognizedĪs a new class of genes involved in human cancer and have recentlyīeen shown to be biomarkers of diagnostic, prognostic and Ranging in size from 19 to 25 nucleotides (nt), is one of the MicroRNAs (miRNAs), a class of short ncRNA molecules Oesophagus and lung, but not detected in normal tissues ( 8, 9). Accordance to the literature, lncRNAīCYRN1is strongly expressed in some carcinomas of the cervic, Brain cytoplasmic RNA 1 (BCYRN1), alsoĬalled BC200, ordinarily cannot be detected in normal tissue,Įxcept for in the primate nervous system ( 7). Up to now, moreĪnd more literature has proved that lncRNA expressions are involved In their infancy and the functions remain unclear. Post-transcriptional level in cellular homeostasis ( 4). Their roles include regulating geneĮxpression at the epigenetic, transcriptional, and LncRNAs are defined as >200 nt in length ( 3). Transcripts are non-coding RNAs (ncRNAs), including lncRNAs. Percent of the human genome, whereas the overwhelming majority of Protein-coding genes account for only about two Therefore, it is very important to studyĪ method to reliably predict disease outcome and reduce risk of Principally because of lacking screening that can prevention andĭetection of early-stage cervical cancer ( 2). The disproportionately high burden of cervical cancer in developingĬountries and elsewhere medically underserved populations is Occurring malignant tumors and the fourth leading cause ofĬancer-related mortality in females worldwide, causing aboutģ00,000 deaths every year. These results suggest that lncRNA BCYRN1 promotes the proliferation and invasion of cervical cancer via targeting miR-138.Ĭervical cancer is one of the most frequently Finally, the in vivo experiment verified that BCYRN1 siRNA was able to prevent tumor growth, and reduced the expression of migration marker proteins metalloproteinase 2 and vascular endothelial cell growth factor, with enhanced expression levels of miR‑138. Additionally, BCYRN1 siRNA partially counteracted the effect of miR‑138 inhibitor on promoting cell viability and mobility in HeLa cells. The targeting association between BCYRN1 and miR‑138 was supported by luciferase reporter assays. Subsequently, elevated levels of miR‑138 were suppressed by transfection with miR‑138 inhibitor in HeLa cells pretreated with BCYRN1 siRNA. Furthermore, microRNA (miR)‑138 was predicted as a direct target of BCYRN1 and the expression of miR‑138 was elevated in HeLa cells transfected with BCYRN1 siRNA. The silence of BCYRN1 significantly reduced cell viability and motility. The expression of BCYRN1 was suppressed following transfection with small interfering RNA (siRNA) in HeLa cells. Elevated levels of BCYRN1 were also detected in three human cervical cancer cell lines (SiHa, HeLa and CaSki) compared with non-cancerous ectocervical epithelial cell line (Ect1/E6E7). The results demonstrated that BCYRN1 was upregulated in cervical cancer tissues compared with normal tissues.

The present study aimed to investigate the effect of BCYRN1 in the viability and motility of cervical cancer, and the relevant mechanism.

High expression of brain cytoplasmic RNA 1 (BCYRN1) has been detected in various tumors. Cervical cancer is one of the most malignant types of tumor and the fourth leading cause of cancer‑associated mortality in females worldwide.

Mir siberian mouse nude